AGM Workshop on DNA-Encoded Libraries for Lead Discovery
Workshop - Day 1 Wed Sep 1, 12:30 PM - 5:00 PM (ET)
Virtual Happy Hour Wed Sep 1, 5:00 PM - 6:00 PM (ET)
Workshop - Day 2 Thu Sep 2, 12:15 PM - 5:15 PM (ET)
About the Workshop
While attention around the use of DNA-Encoded Libraries (DEL) has rapidly grown over the past decade key aspects of DEL design and production, as well as their use for lead discovery, are scarcely detailed in the literature.
NCATS Assay Guidance Manual (AGM) program has taken keen notice of this emerging technology and is hosting a two-day virtual workshop to seed discussion of best practices for using DELs. Many of the instructors are pioneers and experienced developers of the technology, and sessions are aimed at sharing DEL fundamentals to facilitate uptake and learning for both novice and established users. The workshop will include four sessions and feature seminars that highlight critical concepts about the technology including DEL library design & production, affinity selection strategy and execution, sequencing and data analysis; and will close with a panel discussion about emerging trends and technological advances in the DEL field.
Goals and Objectives
The goal of this workshop is to train scientists on the fundamentals and applications of the DEL technology and engage in a dialogue with those curious to understand it on a deeper level. This workshop aims to provide best practices and discuss standards for rigor in the DEL field to enable reproducible results. Through the combined learnings from the four sessions, participants will improve their research projects utilizing DEL for hit discovery and know where to find further information. Novice users will take home practical learnings that can be immediately applied, and experienced practitioners will gain a deeper appreciation for complexities of DEL outside their immediate discipline of expertise. This workshop will also provide participants with a sampling of emerging trends and discuss technological hurdles to the advancement of the field.
About the Assay Guidance Manual
The Assay Guidance Manual (AGM) is a free, best-practices online resource devoted to the successful development of robust, early-stage drug discovery assays.
The manual was originally developed to provide step-by-step guidance based on experiential knowledge from drug developers for planning and executing projects in high-throughput screening, lead optimization and early phases of drug development. The AGM has now been expanded into a unique, user-friendly collection of over 50 chapters of well-tested knowledge, much of which is being documented for the first time. Methods outlined in the manual address appropriate statistical ways to analyze assay results and accommodate minor changes to assay protocols to ensure robustness.
Investigators worldwide can use the manual to design biologically and pharmacologically relevant assays for high-throughput screening and lead optimization to evaluate collections of molecules that modulate the activity of biological targets, pathways and cellular phenotypes.
The National Center for Advancing Translational Sciences (NCATS) manages the content of the manual with input from industry, academia and government experts. More than 100 authors from around the world have contributed content to this free resource, which is updated regularly and housed by the National Library of Medicine. The chapters have PubMed citations for the contributing authors.
Workshop Organizing Committee
Timothy Foley, Pfizer Inc.
Abigail Grossman, NCATS
Matthew Hall, NCATS
Gwenn Hansen, Nurix Therapeutics
David Israel, HitGen Inc.
Lisa Marcaurelle, GlaxoSmithKline
Sarine Markossian, NCATS
Anton Simeonov, NCATS
Kanny Wan, NCATS
- Abigail Grossman